Carbohidrates digestion using usual digestive medicines - a practical class

This work presents a practical class to study the “in vitro” carbohydrates digestion. We used gelatinized starch as substrate and two usual digestive medicines as the amylase source. The enzymatic reactions were made in the dissolutor equipment, temperature 37o C, constant agitation, and buffers to simulate the stomach and small intestine pH. To evaluate the pancreatic enzymes in the medicines, we made an amylase assay using a purified enzyme. The glucose concentration in the reaction middle was measured 0, 30, 60, 90 e 120 min after the starting reaction. These results show differences between the two medicines amylase activity.

Download